Table 1 Name, origin and phytoplasma status of the tissue samples analyzed in this study.

From: Molecular diagnostic assays based on cpn60 UT sequences reveal the geographic distribution of subgroup 16SrXIII-(A/I)I phytoplasma in Mexico

Symptomatic Samples^a	Tissue	Sampled in^b	Phytoplasma status
			PCR^c
			F2nR2^d	cpn60 UT	LAMP^e
Strawberry
2014
S26b-GP-MA	Green petal	M-A	+	+	+
S26b-P-MA	Fruit peduncle	M-A	NT^f,g	NT	+
S26b-F-MA	Fruit pulp	M-A	NT^g	NT	+
S27b-GP-MA	Green petal	M-A	+	+	+
S31b-L-MA	Midrib of green leaves with red margin	M-A	+	+	+
S31b-GP-MA	Green petal	M-A	NT^g	+	+
S31b-P-MA	Fruit peduncle	M-A	NT^g	NT	+
S31b-F-MA	Fruit pulp	M-A	NT^g	NT	+
S267-GP-MA	Green petal	M-A	NT^g	−	−
S267-P-MA	Fruit peduncle	M-A	NT^g	NT	−
S267-F-MA	Fruit pulp	M-A	NT^g	NT	−
S289-L-MA	Midrib of green leaves with red margin	M-A	+	+	+
S289-GP-MA	Green petal	M-A	NT^g	NT	−
S289-P-MA	Fruit peduncle	M-A	NT^g	+	+
S289-F-MA	Fruit pulp	M-A	NT^g	NT	−
2015
S01-L-MA	Midrib of green leaves with red margin	J-A	−	−	−
S01-P-MA	Fruit peduncle	J-A	+	+	+
S02-P-JA	Fruit peduncle	J-A	−	−	−
S03-P-JB	Fruit peduncle	J-B	−	−	−
S04-L-MB	Midrib of green leaves with red margin	M-B	+	+	+
S05-L-MB	Midrib of green leaves with red margin	M-B	+	+	+
S05-P-MB	Fruit peduncle	M-B	+	+	+
S06-L-MB	Midrib of green leaves with red margin	M-B	+	+	+
S06-P-MB	Fruit peduncle	M-B	+	−	+
S07-P-JC	Fruit peduncle	J-C	+	+	+
S08-L-JA	Midrib of green leaves with red margin	J-A	−	−	−
S09-L-MB	Midrib of green leaves with red margin	M-B	+	+	+
S09-P-MB	Fruit peduncle	M-B	+	+	+
S10-L-JC	Midrib of green leaves with red margin	J-C	+	+	+
S10-P-JC	Fruit peduncle	J-C	+	+	+
S11-L-JC	Midrib of green leaves with red margin	J-C	+	+	+
S12-P-JC	Fruit peduncle	J-C	+	+	+
S13-L-JC	Midrib of green leaves with red margin	J-C	+	+	+
S14-P-JA	Fruit peduncle	J-A	−	−	−
S15-L-JA	Midrib of green leaves with red margin	J-A	−	−	+
S16-P-JA	Fruit peduncle	J-A	−	−	−
S17-P-JA	Fruit peduncle	J-A	−	−	−
S18-P-JA	Fruit peduncle	J-A	−	−	−
S19-L-JD	Midrib of green leaves with red margin	J-D	+	+	+
S19-P-JD	Fruit peduncle	J-D	+	+	+
S20-L-JD	Midrib of green leaves with red margin	J-D	−	−	−
S21-L-MB	Midrib of green leaves with red margin	M-B	+	+	+
S21-P-MB	Fruit peduncle	M-B	+	+	+
S22-L-MB	Midrib of green leaves with red margin	M-B	+	−	+
S22-P-MB	Fruit peduncle	M-B	+	+	+
S23-L-MB	Midrib of green leaves with red margin	M-B	+	+	+
S24-P-JB	Fruit peduncle	J-B	−	−	−
S25-L-JC	Midrib of green leaves with red margin	J-C	+	+	+
S25-P-JC	Fruit peduncle	J-C	+	+	+
S26-L-JA	Midrib of green leaves with red margin	J-A	+	+	+
S26-P-JA	Fruit peduncle	J-A	−	−	+
S27-P-JA	Fruit peduncle	J-A	−	−	−
S28-L-MB	Midrib of green leaves with red margin	M-B	+	+	+
S29-L-MB	Midrib of green leaves with red margin	M-B	−	−	−
S30-P-MB	Fruit peduncle	M-B	−	−	−
S31-L-MB	Midrib of green leaves with red margin	M-B	+	+	+
S32-L-MB	Midrib of green leaves with red margin	M-B	+	+	+
S33-L-MB	Midrib of green leaves with red margin	M-B	+	+	+
S34-L-MC	Midrib of green leaves with red margin	M-C	−	−	−
S35-L-MD	Midrib of green leaves with red margin	M-D	−	−	+
S36-L-ME	Midrib of green leaves with red margin	M-E	−	−	+
S37-P-MD	Fruit peduncle	M-D	−	−	−
S38-L-MF	Midrib of green leaves with red margin	M-F	−	−	+
S39-L-MG	Midrib of green leaves with red margin	M-G	−	−	−
S40-L-MH	Midrib of green leaves with red margin	M-H	−	−	−
S41-L-JB	Midrib of green leaves with red margin	J-B	+	+	+
S41-P-JB	Fruit peduncle	J-B	+	+	+
S42-L-JB	Midrib of green leaves with red margin and Fruit peduncle	J-B	+	+	+
S43-L-JB	Midrib of green leaves with red margin	J-B	+	+	+
Periwinkle
2014
P83-L-SLP	Midrib of green leaves	SLP-A	+	+	+
P86-L-SLP	Midrib of green leaves	SLP-A	+	+	+
Raspberry
2015
R01-L-MA	Green leaves	M-A	+	+	+
R01-P-MA	Fruit peduncle	M-A	+	+	+
R02-L-MA	Green leaves	M-A	−	+	+
R03-L-MA	Green leaves	M-A	−	+	+
R04-L-MB	Green leaves	M-B	−	−	+
R05-L-MC	Green leaves	M-C	+	+	+
R06-L-MC	Green leaves	M-C	−	+	+
R07-L-MC	Green leaves	M-C	−	−	+
R08-L-MC	Green leaves	M-C	+	+	+
Blueberry
2015
Bl01-L-JA	Yellow leaves	J-A	+	+	+
Bl02-L-JB	Leaves showing red margin	J-B	−	−	+
Blackberry
2015
Bk01-L-JC	Green leaves	J-C	−	+	+
Bk02-L-JD	Green leaves	J-D	+	+	+
Bk03-L-MD	Green leaves	M-D	−	+	+
Bk04-L-ME	Green leaves dry	M-E	−	−	−

^aSymptoms observed in strawberry and periwinkle plants were previously described³⁰. Blackberry and raspberry plants showed small leaves and green structures in the fruits; blueberry plants showed yellow leaves and leaves with red margins (Figure S1). ^bMichoacan samples: 20.002°N, 102.3089°W; San Luis Potosi samples: 22.2°N, 100.1°W; Jalisco samples: 20.34°N 103.41°W. A total of 11 commercial farms located among the three states mentioned above were visited to collect the symptomatic samples. ^cPCR using primers R16F2n/R16R2³¹ to amplify the F2nR2 sequence and H279p/H280p¹² to amplify the cpn60 UT sequence. ^dNested PCR was performed on the samples collected in 2015, with P1/P7 primers in the first reaction^{32, 33}, and R16F2n/R16R2 in the nested reaction³¹. Samples collected in 2014 were analyzed with direct PCR targeting the F2nR2 fragment. ^eLAMP using primers and conditions described in Table 1. ^fNT, not tested. ^gSample was negative using 16S-targeted primers P1/Tint⁸.

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Table 1 Name, origin and phytoplasma status of the tissue samples analyzed in this study.

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