Table 3 Molecular quantification of SbGP/MPV phytoplasma in infected plant samples obtained in 2014.

From: Molecular diagnostic assays based on cpn60 UT sequences reveal the geographic distribution of subgroup 16SrXIII-(A/I)I phytoplasma in Mexico

Sample

Method Unit

qRT-PCR genomes/g tissue

ddPCR genomes/g tissue

LAMP Time to positive (Tp), minutes (calcein detection)

Tissue

Mean (n)

Standard deviation

Mean (n)

Standard deviation

Mean (n)

Standard deviation

S31b-L-MA

Leaf midrib

4.82 × 108 (10)

5.48 × 107

2.41 × 108 (10)

2.51 × 107

45.88 (2)

3.01

S31b-GP-MA

Green petal

2.74 × 109 (2)

7.58 × 107

4.80 × 108 (2)

4.88 × 107

31.88 (2)

3.00

S31b-P-MA

Fruit peduncle

4.34 × 108 (2)

2.01 × 107

2.11 × 108 (2)

2.61 × 107

35.0 (3)

0.43

S31b-F-MA

Fruit pulp

9.69 × 107 (2)

1.80 × 107

5.48 × 107 (2)

4.24 × 106

45 (2)

3.54

S289-L-MA

Leaf midrib

1.34 × 108 (10)

1.78 × 107

7.94 × 107 (9)

1.14 × 107

49.63 (2)

3.01

S289-GP-MA

Green petal

NDa (2)

 

ND (2)

 

ND (2)

 

S289-P-MA

Fruit peduncle

4.24 × 108 (2)

1.29 × 107

7.16 × 107 (2)

3.61 × 106

35.38 (2)

0.18

S289-F-MA

Fruit pulp

ND (2)

 

ND (2)

 

ND (2)

 

S26b-GP-MA

Green petal

1.12 × 109 (2)

7.44 × 107

3.85 × 108 (2)

7.64 × 106

35.38 (2)

1.24

S26b-P-MA

Fruit peduncle

1.04 × 109 (2)

2.90 × 108

4.23 × 108 (2)

6.36 × 106

25.25 (2)

0

S26b-F-MA

Fruit pulp

3.98 × 108 (2)

1.22 × 107

1.46 × 108 (2)

1.09 × 107

38.38 (2)

0.18

S27b-GP-MA

Green petal

4.01 × 109 (2)

4.07 × 108

6.92 × 108 (2)

1.23 × 108

30.88 (2)

1.59

S267-GP-MA

Green petal

8.21 × 102 (4)

4.53 × 102

ND (2)

 

ND (2)

 

S267-P-MA

Fruit peduncle

ND (2)

 

ND (2)

 

ND (2)

 

S267-F-MA

Fruit pulp

ND (2)

 

ND (2)

 

ND (2)

 

P83-L-SLP

Leaf midrib

4.67 × 109 (10)

7.72 × 108

2.53 × 109 (10)

1.80 × 108

38.42 (3)

2.31

P86-L-SLP

Leaf midrib

6.13 × 109 (10)

8.46 × 108

2.82 × 109 (10)

3.10 × 108

37.08 (3)

2.13

Strawberry (H)b

Leaf

ND (2)

 

ND (2)

 

ND (2)

 

Periwinkle (H)

Leaf

ND (2)

 

ND (2)

 

ND (2)

 

Raspberry (H)

Fruit

ND (2)

 

ND (2)

 

ND (2)

 

Blueberry (H)

Leaf

ND (2)

 

ND (2)

 

ND (2)

 

Blackberry (H)

Fruit

ND (2)

 

ND (2)

 

ND (2)

 

Nontargetc

 

ND (2)

 

ND (2)

 

ND (2)

 
  1. aND, not detected. bH, DNA from uninfected plant. See Supplementary Fig. S4 for results. cNontarget DNA is a mixture of plasmids consisting of 106 copies each of all of the cpn60 UT fragments from the phytoplasma strains described in Dumonceaux et al.12. See Supplementary Fig. S4 for results on individual templates.
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