Table 1 Binding affinity toward Trp, coupling efficiency, NADPH consumption and total turnover numbers of TxtE and its chimeras.
From: Engineered P450 biocatalysts show improved activity and regio-promiscuity in aromatic nitration
Chimeras | Kd (µM) | Coupling (%) | NADPH consumption (µM/min) | TTN |
|---|---|---|---|---|
TxtE | 24.8 ± 1.1 | 2.4 ± 0.3 | 42.3 ± 4.2 | 378 ± 17 |
TB11 | 16.2 ± 0.6 | 5.2 ± 0.5 | 32.8 ± 5.0 | 535 ± 28 |
TB12 | 22.3 ± 1.4 | 1.8 ± 0.2 | 86.3 ± 3.9 | 332 ± 40 |
TB13-Q | 21.5 ± 0.8 | 2.0 ± 0.1 | 73.9 ± 3.8 | 308 ± 18 |
TB13S | 19.3 ± 0.6 | 2.6 ± 0.2 | 30.8 ± 3.1 | 202 ± 20 |
TB13 | 21.3 ± 0.4 | 2.2 ± 0.3 | 65.7 ± 4.1 | 342 ± 29 |
TB14 | 17.4 ± 1.0 | 5.3 ± 0.5 | 45.5 ± 4.8 | 707 ± 16 |
TB15 | 21.4 ± 0.9 | 3.9 ± 0.4 | 53.6 ± 5.1 | 658 ± 33 |
TB16 | 18.9 ± 1.0 | 2.7 ± 0.2 | 80.5 ± 4.3 | 351 ± 39 |
TB17 | 15.5 ± 0.6 | 2.1 ± 0.3 | 50.1 ± 4.6 | 348 ± 24 |
TB19 | 16.0 ± 0.8 | 2.4 ± 0.5 | 52.1 ± 6.7 | 464 ± 21 |
TB22 | 27.1 ± 1.3 | 2.4 ± 0.5 | 46.3 ± 6.3 | 408 ± 32 |
TB24 | 14.9 ± 0.3 | 2.3 ± 0.1 | 46.4 ± 3.4 | 381 ± 15 |
TB27 | 16.2 ± 1.1 | 2.0 ± 0.6 | 51.1 ± 7.2 | 548 ± 17 |
