Figure 5
From: Biochemistry of malaria parasite infected red blood cells by X-ray microscopy
Principle of X-ray fluorescence imaging illustrated by a malaria parasite infected red blood cell (RBC). The RBC cytosol (RBCcyto), parasite (Pcyto) and inside the parasite the digestive vacuole (DV) are shown in orange, blue and green respectively. The vitrified ice of thickness d is between the two blue lines at angle π/2 − α relative to the beam. The sample is partitioned into voxels given by the integer coordinates \((i,j,k)\) with k being in the direction of the incident X-ray beam. Three positions of i in the scan of the sample are shown by the red arrows denoting the X-ray beam: one traverses only the vitrified ice, the next only the cytosol of the infected RBC, and the third traverses also the parasite cytosol. The relevant path lengths in the different compartments are derived from the cryo soft X-ray tomography. The fluorescent X-rays are shown by the dashed arrows.
