Figure 6 | Scientific Reports

Figure 6

From: Modulation of glucose metabolism by a natural compound from Chloranthus japonicus via activation of AMP-activated protein kinase

Figure 6

Effect of shizukaol F on the energy status and respiration. (A) Dose-dependent depolarization of mitochondrial membrane potential in C2C12 cells for 10 min of shizukaol F treatment at the indicated concentrations. 10 μM CCCP was set as positive control. **P < 0.01 (one way ANOVA). n = 3 independent biological replicate experiments. (B) Dose-dependent increase of the AMP/ATP ratio in myotubes exposed to shizukaol F at the indicated concentrations for 2 h. *P < 0.05; **P < 0.01 (one way ANOVA). n = 3 independent biological replicate experiments. (C) Time-dependent stimulation of the AMP/ATP ratio in myotubes by 1 μM shizukaol F. **P < 0.01 (one way ANOVA). n = 3 independent biological replicate experiments. (D) Inhibition of the respiration of intact C2C12 cells by shizukaol F treatment at the indicated concentrations (n = 3). *P < 0.05; **P < 0.01 (one way ANOVA). (E) Effect of shizukaol F on the respiration of isolated mitochondria from mice liver (n = 3). *P < 0.05; **P < 0.05 (one way ANOVA). (F) Lactate concentrations of C2C12 cells treated with shizukaol F at indicated conditions were measured. Rosiglitazone (50 μM) was set as a positive control. *P < 0.05; **P < 0.01 (two way ANOVA). n = 3 independent biological replicate experiments.

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