Figure 4
From: Granulosa cell endothelin-2 expression is fundamental for ovulatory follicle rupture
Esr2-Edn2KO mice have more antral follicles and fewer corpora lutea after ovulation. (A) Taqman quantitative RT-PCR results comparing Edn2 expression in WT and Esr2-Edn2KO mice at hCG12 hours; 97.6% of Edn2 expression is lost. (B) ELISA quantitation of total Endothelin protein per ovary. Endothelin mass is significantly reduced at hCG12 hours in Esr2-Edn2KO mice and is not different at hCG0 hours from WT mice. (C) Serum was extracted from PND25 mice at hCG24 hours and serum hormone concentrations (ng/mL) were quantified by LC/MS/MS. P-values represent ANOVA score by hormone. Error bars represent the SEM. (D) Ovaries were collected at hCG24 hours from PND25 mice, fixed, and serially sectioned. The absolute number of corpora lutea (CL), antral follicles (AF), and follicles with hemorrhage present were counted. The value is also expressed as a percentage of total ovarian structures. Error bars represent the SEM.
