Figure 5
From: Granulosa cell endothelin-2 expression is fundamental for ovulatory follicle rupture
Ablation of Ednra in granulosa cells reduces ovulation. Ednra-flox mice were bred with Esr2-iCre mice to produce Esr2-EdnraKO mice. (A ) After superovulation, oocytes were collected and counted at hCG24 hrs. Esr2-EdnraKO mice ovulated significantly fewer oocytes than WT controls, and had a greater range of oocytes ovulated. Error bars represent the SEM; diamonds indicate each data point. (B) Histology of representative ovaries at hCG24 hrs from each genotype, 4X. (C) WT and Esr2-EdnraKO female mice were bred with control proven males, one male and one female of each genotype per cage for 10 days. The percentage of females that gave births and the number of pups per litter quantified. There was no difference between groups. (D) Histology of representative ovaries after breeding study, 4x. Multiple corpora lutea were present in each group.
