Figure 6
From: Granulosa cell endothelin-2 expression is fundamental for ovulatory follicle rupture
EDN2 induces contraction in mouse ovaries and uteri in an Endothelin receptor-dependent manner. WT mice were superovulated and ovaries (n = 8) were collected at hCG12–16 hours and were placed in a myograph machine for tension analysis. (A) A representative control ovary response to a 60 mM K + PSS solution (for normalization), a 50 nM EDN2 solution, and a 140 nM tezosentan solution to block Endothelin receptors. This treatment schema was similarly used to measure responses in (B) WT uteri (n = 5). Vertical axis: tension in millinewtons where 9.81 mN = 1.00 gram-force. Horizontal axis: Time (Hours:Minutes:Seconds). (C) Comparison of absolute tension changes between tissues in response to Endothelin receptor agonization and subsequent antagonization. (D) Ovarian and uterine contraction normalized to the response to K + PSS to compare relative contractile responses. Error bars represent the SEM.
