Figure 5 | Scientific Reports

Figure 5

From: LPS promotes a monocyte phenotype permissive for human cytomegalovirus immediate-early gene expression upon infection but not reactivation from latency

Figure 5

The supernatant of latently infected monocytes does not prevent LPS mediated effects on uninfected monocytes. To generate a latent secretome monocytes were infected with HCMV and cell free conditioned media harvested at 7dpi (s/n). Fresh monocytes were either incubated with normal (mono) or conditioned (mock + s/n) media for 48 hours. A fraction of the cells from both conditions were then further stimulated with (500 ng/ml LPS for 24 hours). Alternatively, uninfected monocytes were differentiated to permissive dendritic cells and incubated with normal media (iDC) or supernatant from latently infected monocytes (iDC + s/n) again for 48 hours. All cells were then infected with Merlin 2 days post incubation with mock or conditioned media and stained for IE protein expression 24 hpi. Nuclei were counter-stained with DAPI and infection rate calculated n = 3. NS = non-significant difference when comparing infection rates between mock and conditioned media treated cells.

Back to article page