Figure 6 | Scientific Reports

Figure 6

From: LPS promotes a monocyte phenotype permissive for human cytomegalovirus immediate-early gene expression upon infection but not reactivation from latency

Figure 6

Incubation of monocytes with LPS promotes a unique phenotype. CD14+ monocytes were isolated by magnetic cell separation using CD14 beads from PBMC. Monocytes were incubated in media or stimulated with 500 ng/ml LPS for 1 and 3 days, IL4/GMCSF or MCSF for 6 days. Post incubation, monocytes were stained with cell surface antibodies to various phenotypic markers (CD14, CD83, CD86, CCR5, MHC Class II (HLA-DR), CD11b, CD209 (DC-SIGN), MHC Class I, CD80 or appropriate Isotype controls. Flow cytometry histogram plots against isotype controls for each treatment condition and phenotypic marker are shown.

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