Figure 7 | Scientific Reports

Figure 7

From: LPS promotes a monocyte phenotype permissive for human cytomegalovirus immediate-early gene expression upon infection but not reactivation from latency

Figure 7

The LPS induced permissiveness of monocytes is transient. (A) Monocytes (monos) were either differentiated to dendritic cells (iDCs), stimulated with 500 ng/ml LPS for 72 hours (mono-LPS) or stimulated with LPS for 24 hours and then washed for 48 hours. Cells were then infected with Merlin and stained for IE protein expression 24 hpi. Nuclei were counter-stained with DAPI and infection rate calculated n = 3. (B) Monocytes were stimulated with 500 ng/ml LPS and then either incubated for 72 hours (Mono-LPS) or incubated for 24 hours followed by 48 hours in media alone (Mono LPS washed). DNA was then harvested and amplified in a diagnostic qPCR against gB with copy number normalised to cell number using a beta globin PCR n = 3. **p < 0.01 comparing infection in LPS and LPS-washed cells.

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