Figure 8 | Scientific Reports

Figure 8

From: LPS promotes a monocyte phenotype permissive for human cytomegalovirus immediate-early gene expression upon infection but not reactivation from latency

Figure 8

A COX-2 inhibitor antagonises LPS mediated permissiveness of monocytes. (A) Monocytes were incubated with control (DMSO), or inhibitors of ERK, COX2, p38 or JNK for 4 hours prior to addition of LPS (500 ng/ml). After 24 hours, cells were then infected with Merlin and stained for IE protein expression 24hpi. Nuclei were counter-stained with DAPI and infection rate calculated n = 3. **p < 0.01; NS = non-significant difference when compared to infection of DMSO solvent control. (B) HFFs, monocyte derived dendritic cells (DCs) or epithelial cells (ARPE) were pre-treated with DMSO or a COX-2 inhibitor for 4 hours then infected with Merlin and stained for IE protein expression 24hpi. Nuclei were counter-stained with DAPI and infection rate calculated n = 3. NS = non-significant difference when comparing rate of infection of each cell type plus and minus inhibitor.

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