Figure 2 | Scientific Reports

Figure 2

From: The glycogen synthase kinase MoGsk1, regulated by Mps1 MAP kinase, is required for fungal development and pathogenicity in Magnaporthe oryzae

Figure 2

M. oryzae MoGSK1 can functionally complement the Δmck1 mutant of S. cerevisiae. Yeast strains were grown at 12 °C for 14 days on galactose or glucose-supplemented YP medium. Plates were inoculated with 10 μl droplets containing 1 × 106, 5 × 105, 1 × 105, 5 × 104, or 1 × 104 cells/ml and left to grow for 14 days before examination. Triangles indicate the decreasing concentration of yeast cells. BY1471 (WT) is the wild type S. cerevisiae strain. Δmck1 is the yeast mutant sensitive to cold condition. The Δmck1:MoGSK1 transformant is expressing the M. oryzae MoGSK1 under the S. cerevisiae galactose-inducible GAL1 promoter. The negative control is the Δmck1 mutant transformed with the empty pYES vector.

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