Figure 4 | Scientific Reports

Figure 4

From: The glycogen synthase kinase MoGsk1, regulated by Mps1 MAP kinase, is required for fungal development and pathogenicity in Magnaporthe oryzae

Figure 4

Plant infection assays and microscopic observation on infection process of the Δmogsk1 mutant. (A) Appressoria of Ku80 and Δmogsk1 were induced at the hyphal tips following 48 h inoculation on hydrophobic cover slips at a moisture chamber at room temperature. Bar = 10 μm. (B) Microscopic observation on mycelial plug inoculated area on unwounded barley leaf tissues at 48 hr post inoculation. Bar = 10 μm. (C) Equal amounts of mycelial plugs from Ku80, Δmogsk1 and Δmogsk1/MoGSK1 were inoculated on 15-day-old rice seedlings (CO39). Photos were taken post 5-day inoculation. (D) Disease symptoms on wounded and unwounded 7-day-old susceptible barley seedlings induced by mycelia plugs of Ku80 and Δmogsk1 were photographed post 5-day inoculation.

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