Figure 5

Subcellular localization and complementation of MoGsk1 and Fgk3 in M. oryzae. (A) Colonies of Ku80, Δmogsk1, Δmogsk1/MoGSK1-GFP and Δmogsk1/Fgk3-GFP grown on CM medium at 26 °C. Photos were taken at 7 days after inoculation. (B) Equal amounts of mycelial plugs from Ku80, Δmogsk1, Δmogsk1/MoGSK1-GFP and Δmogsk1/Fgk3-GFP were inoculated on 7-day-old barley seedlings to observe pathogenic development. Photos were taken post 5 days inoculation. (C) Conidia from transformants Δmogsk1/MoGSK1-GFP and Δmogsk1/Fgk3-GFP were examined by epifluorescence (GFP) microscopy. Bar = 10 μm.