Figure 4
From: Poly-protein G-expressing bacteria enhance the sensitivity of immunoassays
The antibody-trapping ability of poly-protein G-expressing bacteria. (a) Equivalent amounts of BL21, BL21/1G and BL21/8G cells were coated on 96-well plates. The absorbance of the bacteria was measured at 600 nm. (b) The antibody-trapping ability of BL21, BL21/1G and BL21/8G cells coated on 96-well plates was analyzed by HRP-conjugated goat antibody. Statistical analysis was performed by one-way ANOVA. (c) BL21/1G and BL21/8G cells were preserved in PBS containing 30% (v/v) glycerol at indicated temperatures for 40 days. The antibody-trapping ability of these bacteria was analyzed by HRP-conjugated goat antibody. Statistical analysis was performed by two-way ANOVA. Bar, SD. ns, no significant difference. *P value < 0.05; **P value < 0.001.
