Figure 5
From: Poly-protein G-expressing bacteria enhance the sensitivity of immunoassays
ELISA tests performed in the presence and absence of poly-protein G-expressing bacteria. Anti-PEG antibody (6.3-biotin) mixed with BL21/1G cells or BL21/8G cells, or not mixed with anything, was added into 96-well plates coated with (a) PEG5k-BSA or (b) BSA. The binding amount of 6.3-biotin was determined by measuring absorbance at 405 nm after staining with SA-HRP and ABTS. Ab, 6.3-biotin. Statistical analysis was performed by one-way ANOVA. Bar, SD. ns, no significant difference. **P value < 0.001.
