Figure 6
From: Poly-protein G-expressing bacteria enhance the sensitivity of immunoassays
Detection of IFN-α and Pegasys by traditional sandwich ELISA or BL21/8G-based sandwich ELISA. (a) Serial diluted IFN-α samples were added into 96-well plates coated with anti-IFN-α capture antibody (MT1, IgM). Captured IFN-α molecules were detected by biotin-conjugated anti-IFN-α detection antibody (MT2, IgG) mixed with BL21/8G cells or not mixed with BL21/8G cells, and then color was developed using ABTS. (b) Serially diluted Pegasys samples were added into 96-well plates coated with anti-PEG capture antibody (AGP4, IgM). Captured Pegasys molecules were detected by biotin-conjugated anti-PEG detection antibody (3.3-biotin, IgG) mixed with BL21/8G cells or not mixed with BL21/8G cells, and color was developed using ABTS. Statistical analysis was performed by an independent t-test. Bar, SD.
