Figure 4
Up-regulation of miRNA-29c and inflammatory cytokines and down-regulation of TTP in podocytes. (A) Real-time qPCR analysis shows miRNA-29c expression in podocytes treated with high glucose (25 mM) for 6 h as com-pared with podocytes treated with either normal glucose (5.6 mM) or mannitol (25 mM). Measured transcript levels were normalized to U6 snRNA expression. (B) Podocytes were cultured in high glucose for 24 h as compared with normal glucose or mannitol, TTP protein expression was assessed by Western blot, GAPDH served as an internal control. (C) The quantified Western blot data shown in graph format. (D) qRT-PCR analysis of TTP mRNA expression in hyperglycemic condition. (E) IL-6 and TNF-α protein expression by ELISA analysis in hyperglycemic condition for 48 h. (F) qRT-PCR analysis of TTP mRNA expression in hyperglycemic condition. Results were obtained from three independent experiments. Data are shown as mean ± SEM (n = 3). *P < 0.05 compared with control.
