Table 2 Bioassay of extracts from Nigerian propolis against T. brucei and C. fasciculata.

From: The Chemical Characterization of Nigerian Propolis samples and Their Activity Against Trypanosoma brucei

 

T. brucei s427

T. brucei B48

T. brucei aqp2/aqp3 null

C. fasciculata (ATCC 50083)

Sample

EC50 1

SEM

EC50*

SEM

RF

ttest3

EC50*

SEM

RF

ttest3

EC50 2

SEM

S95N

17.3

0.24

29.3

1.46

1.69

<0.01

17.9

0.12

1.03

0.10

21.3

1.6

S96N

23.4

0.77

31.4

1.09

1.34

<0.01

23.8

0.56

1.02

0.68

37.5

2

S97N

15.5

0.20

18.2

1.81

1.18

0.21

13.5

0.27

0.87

<0.001

ND

ND

P3N

32.6

0.33

47.2

3.91

1.45

0.02

40.6

2.48

1.25

0.03

58.4

2.4

KK-N

63.7

0.51

74.7

2.68

1.17

0.02

53.4

3.20

0.84

0.03

30.6

1.9

Kap-N

7.8

0.41

9.5

0.77

1.21

0.14

10.1

0.31

1.28

0.01

9.5

0.4

AF23N

inactive

 

Inactive

inactive

>200

 

CCN

9.1

0.17

10.8

0.80

1.19

0.10

8.7

0.20

0.95

0.19

32.7

0.95

BRN

6.9

0.30

7.2

0.28

1.04

0.58

NA

25.9

0.55

RSN

4.2

0.04

3.9

0.16

0.92

0.13

4.4

0.26

1.05

0.46

1.2

0.1

ION

5.9

0.02

6.0

0.21

1.01

0.73

5.2

0.10

0.88

0.00

17.2

0.75

UDN

12.1

0.32

12.6

0.08

1.04

0.35

 

NA

  

7.6

0.65

Pentamidine

0.0023

0.0002

0.5

0.04

225

<0.001

0.1

0.002

31.08

<0.001

18.3

1.9

Menadione

—

—

—

—

—

—

—

—

—

—

0.8

0.1

  1. EC50 values are expressed in µM for pentamidine, and as µg/mL for the extracts, and represent the average and SEM of 3 or 4 experiments. 1n = 3; 2n = 4; 3unpaired two-tailed Student’s t-test comparing EC50 value of the resistant strain with that of the same sample for the control strain s427. RF: resistance factor, being the EC50 value for the resistant strain divided by the EC50 value for the control (sensitive) strain. ND = not determined.
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