Figure 3
Comparative analysis of IncI2 plasmids pMRY13-131_2, pMRY15-117_2 and pMRY16-002_4. (A) Schematic comparative analysis of whole IncI2 plasmid sequences. The red and blue bars between chromosomal DNA sequences represent individual nucleotide matches in the forward and reverse directions, respectively. (B) Schematic representation and comparison of shufflon regions and flanking regions. Six coloured arrows (orange, sky blue, purple, light green, red and blue) represent the 3′ ORF region of the pilV gene in IncI2 plasmid shufflon segments. A grey arrow and black box represent the rci gene and main pilV gene, respectively. (C) Multiple alignments of six repeat regions of shufflon segments. These repeat regions were analysed by the sequence logo program. (D) Identical ratio plot of the 3′-end of shufflon regions and minimum spanning network analysis of SNVs in 23-bp conserved repeat regions. The numbers on the edges of the network show the number of SNVs between the repeat sequences.
