Table 1 General features of three isolates and their complete genome sequences.

From: Elucidation of quantitative structural diversity of remarkable rearrangement regions, shufflons, in IncI2 plasmids

Strain name

Organism

Country

Isolation source

Year

In silico serotyping

Replicon

Chromosomal sequence type or plasmid Inc type

Drug resistance genes

Length (bp)

Average coveragea

Copy numberb

MRY16-002 (=20Ec-P-124)

E. coli

Japan

swine

2008

O24:H4

chromosome

ST117

N.D.

4,920,828

135.41

1.0

pMRY16-002_1

IncFIB and X1

N.D.

168,972

185.91

1.4

pMRY16-002_2

N.D. (phage-like plasmid)

N.D.

108,986

298.92

2.2

pMRY16-002_3

IncP

aph(3′)-Ia, tet(A)

108,957

176.26

1.3

pMRY16-002_4

IncI2

mcr-1

61,805

219.75

1.6

pMRY16-002_5

Col156

N.D.

6,078

N.A.

N.A.

pMRY16-002_6

N.D.

N.D.

4,073

N.A.

N.A.

MRY15-117

E. coli

Japan

cattle

2012

O11:H25

chromosome

ST457

sul2, strA, strB, tet(A), floR

5,117,319

164.98

1.0

pMRY15-117_1

IncFIB and FII

dfrA14, mph(A), erm(B), aac(3)-IIa, ∆bla TEM-1, bla CTX-M-27

116,529

269.38

1.6

pMRY15-117_2

IncI2

mcr-1

61,223

221.03

1.3

MRY15-131

E. coli

Japan

cattle

2013

O1:H25

chromosome

ST457

sul2, strA, strB, tet(A)

5,042,704

135.07

1.0

pMRY15-131_1

IncFIB and FII

dfrA14, mph(A), erm(B), aac(3)-IIa, ∆bla TEM-1, bla CTX-M-27

116,529

218.3

1.6

pMRY15-131_2

IncI2

mcr-1

60,722

187.18

1.4

  1. aThe average coverage of complete sequences was calculated by PacBio raw-read mapping analysis using the SMRT portal software.
  2. bThe copy number was calculated by the number of plasmid coverage divided by that of chromosomal coverage.
  3. ST, sequence type; bp, base pair; N.D., not detected; N.A., not available.