Table 2 Primer and VNTR loci information for the MLVA-13Bc genotyping method developed in this study.
Reactiona | VNTR locusb | CHRc | Forward Primer | Reverse Primer | Annealing Temperature | Repeat Length (bp) | No. of Repeat | Amplicon Length (bp) | Location on Genomec | Reference |
|---|---|---|---|---|---|---|---|---|---|---|
1 | BCTR06 | I | CCTTGACTGAAAAAGGGTGATT | AGAAGGCACTCGATCTCATCG | 58 °C | 8 | 3–15 | 119–215 | 734705–734855 | This study |
BCTR09 | II | GCTTTATCTTATCTGATTCTTTCAAATTC | GCTGACAGAACCCATCGTCAAT | 58 °C | 8 | 9–25 | 241–369 | 73060–73316 | This study | |
2 | Bruce07 | I | GCTGACGGGGAAGAACATCTAT | ACCCTTTTTCAGTCAAGGCAAA | 58 °C | 8 | 1–7 | 142–190 | 734557–734722 | |
BCTR12 | II | GATCCTGATCGTTCGCTTCG | CGTCGATGCACGGACTATCG | 58 °C | 8 | 4–17 | 402–506 | 975304–975737 | This study | |
3 | BCTR03 | I | AAGCGGCGAGAGTTTGTCGT | GATGAAGTCGTTGCTGCCTATTAC | 58 °C | 8 | 5–20 | 409–529 | 86891–87307 | This study |
Bruce16 | II | ACGGGAGTTTTTGTTGCTCAAT | GGCCATGTTTCCGTTGATTTAT | 58 °C | 8 | 1–12 | 144–232 | 748957–749140 | ||
4 | BCTR02 | I | GGAACCCGACAGTGAACACG | ATAATCTCGACACGCAGGCAAC | 60 °C | 8 | 1–23 | 346–522 | 64479–64896 | This study |
Bruce09 | I | GCGGATTCGTTCTTCAGTTATC | GGGAGTATGTTTTGGTTGTACATAG | 60 °C | 8 | 2–16 | 124–236 | 1398399–1398578 | ||
5 | BCTR01 | I | GTTTTTGGTTGCGCATGG | GGTGGATTTCAGGAACAACG | 62 °C | 8 | 8–24 | 214–342 | 62690–62943 | This study |
BCTR08 | II | GTCTGCTGCACTCAAGACGATC | GAATTTGAAAGAATCAGATAAGATAAAGC | 62 °C | 8 | 2–10 | 182–246 | 72899–73088 | This study | |
6 | Bruce04 | I | CTGACGAAGGGAAGGCAATAAG | CGATCTGGAGATTATCGGGAAG | 62 °C | 8 | 2–7 | 160–200 | 438775–438966 | |
BCTR11 | II | CAGAATTTTCGAGGCATTCG | GAGACGGTGGGGGTTAAAG | 62 °C | 8 | 3–13 | 195–275 | 969122–969364 | This study | |
7 | Bruce18 | II | TATGTTAGGGCAATAGGGCAGT | GATGGTTGAGAGCATTGTGAAG | 60 °C | 8 | 2–6 | 138–170 | 958389–958534 |
