Figure 7
Validation of Tn mutant library screen during adhesion and invasion of human gut epithelial tissue culture cells. Adhesion to (a), and invasion (b) of, Caco-2 cells by C. jejuni M1cam defined gene deletion mutants. Mutants in luxS and pstA, which were not identified in our Tn-seq screen with Caco-2 cells, were included as negative controls and rpoN, which was previously shown to be required for adhesion and invasion81, 82, served as a positive control. Caco-2 adhesion (c), and invasion (d), of gene deletion and genetically complemented mutants. Data is represented as percentage of wild-type (n ≥ 3) and plotted as means and SEM. The competitive index (CI) was calculated by dividing the ratio of mutant to wild-type bacteria recovered upon (e) adhesion to, and (f) invasion of, Caco-2 cells by the ratio of mutant to wild-type bacteria that were used in the inoculum. Statistical significance was calculated using a Mann-Whitney test where *P < 0.05, **P < 0.01 and ***P < 0.001.
