Figure 4

Esg and Sc antagonize to regulate Pros expression. (a,b) Esg overexpression cannot rescue ISC loss and ee cell specification caused by Pros overexpression. Clones in UAS-pros and UAS-esg & -pros lines were induced by Flp-Out (F/O) system, and samples were stained with DAPI (blue) and dTK (red). Scale bars 20 μm. (c–e) Knocking down pros rescues the increase of ee cell number caused by esg knockdown. Esg-Gal4 drives UAS-GFP alone (control), esg-RNAi, and esg- & pros-RNAi at 29 °C for 5 days and samples were stained with DAPI (blue) and dTK (red). Scale bars 50 μm. (f) Quantification of dTK⁺ cells in images (c,d, and e). (control, n = 3; esg-RNAi, n = 7; esg- & pros-RNAi, n = 4. Students’ t test with Welch’s correction, **(left, p = 0.0042), **(right, p = 0.0049)). (g–i) Sc overexpression partially rescues Pros expression that repressed by Esg overexpression. Rab3-Gal4 drives the expression of UAS-RFP alone (control), UAS-esg, and UAS-esg & -sc. Samples were stained with DAPI (blue) and Pros (green). The white arrowheads point to RFP⁺ Pros⁻ cells. (j) Quantification of RFP⁺ Pros⁻/RFP⁺ cells in images (g,h,i). Students’ t test, ***(p < 0.0001), *(p = 0.0417).