Figure 1
From: COX-1/PGE2/EP4 alleviates mucosal injury by upregulating β-arr1-mediated Akt signaling in colitis
Expression of COX, prostaglandins and prostaglandin receptors in colitis. (A) COX-1 and COX-2 expression in colon tissues were determined using RT-PCR and Western blotting in the non-UC group (human normal colon tissue), injury group (colitis colon tissue in the injury phase) and repair group (colitis colon tissue in the repair phase); β-actin was used as a loading control (n = 6 per group). *P < 0.05, # P < 0.05 non-UC versus injury. (B) COX-1 and COX-2 expression in mouse colon tissue was determined using RT-PCR and Western blotting analyses in the vehicle group, injury group and repair group. (n = 4 per group) *P < 0.05, # P < 0.05 vehicle versus injury (C) Concentrations of mucosal prostaglandins PGE2, PGD2, PGF2α and PGI2 in of three pairs of representative human specimens. Values are expressed as the mean ± SD (n = 6 per group). *P < 0.05, non-UC versus injury phase. # P < 0.05 injury phase versus repair phase (D) mRNA expression of EP1, EP2, EP3 and EP4 in the colonic mucosa were evaluated using real-time PCR in the indicated group. Values are expressed as the mean ± SD (n = 6 per group). *P < 0.05, non-UC versus injury phase. # P < 0.05 injury phase versus repair phase (E) Concentrations of mucosal prostaglandins PGE2, PGD2, PGF2α and PGI2 in the mouse vehicle group, injury group and repair group; The values are expressed as the mean ± SD (n = 4 in each group). *P < 0.05 versus vehicle mice, # P < 0.05 injury group versus repair group. (F) EP1, EP2, EP3 and EP4 mRNA expression in the colonic mucosa of mice were evaluated by real-time PCR in the indicated group. The values are expressed as the mean ± SD (n = 4 in each group). *P < 0.05 versus vehicle mice, # P < 0.05 injury group versus repair group.
