Figure 2
Effects of FLX on labelled bile acids and CDF clearance in HepaRG cells and PHH. (A) NBD-UDCA and CDF efflux in HepaRG hepatocytes and PHH treated for 2 h with different concentrations of FLX (0–6 mM). Orange arrows indicate fluorescence in BC (bar = 50 μm). (B) Quantification of CDF accumulation in BC of HepaRG hepatocytes after FLX treatment using ImageJ 1.48 software as described in the Materials and Methods. (C) [3H]-TA clearance in HepaRG cells treated with different concentrations of FLX (0–8 mM) for 2 h. Data were expressed relative to those of untreated cells arbitrarily set at 100%. They represent the means ± SEM of 3 independent experiments. *p < 0.05 compared with that of untreated cells.
