Figure 3 | Scientific Reports

Figure 3

From: Activated hepatic stellate cells secrete periostin to induce stem cell-like phenotype of residual hepatocellular carcinoma cells after heat treatment

Figure 3

POSTN promoted the stemness of heat-exposed residual HCC cells via integrin β1/AKT/GSK-3β/β-catenin/TCF4/Nanog signaling pathway. Western blots were performed. (a) POSTN upregulated the phosphorylation level of AKT and GSK-3β, and the expression of nuclear β-catenin, TCF4 and Nanog in heat-exposed residual MHCC97H and Huh7 cells. The levels of GSK-3β phosphorylation, β-catenin, TCF4 and Nanog expression were reduced by the administration of GSK-3β inhibitor CHIR 99021. (b) Integrin β1 knockdown attenuated POSTN-induced levels of AKT and GSK-3β phosphorylation, β-catenin, TCF4 and Nanog expression in heat-exposed residual MHCC97H cells.

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