Figure 4 | Scientific Reports

Figure 4

From: A role of human RNase P subunits, Rpp29 and Rpp21, in homology directed-repair of double-strand breaks

Figure 4

Rpp29 and Rpp21 bind poly(ADP-ribose) (PAR) in vitro. (A) Protein alignment reveals that Rpp29 and Rpp21 contain a PAR-binding consensus (bold letters). (B) PAR-binding assay with full-length, 6 × His-tagged recombinant Rpp29 and Rpp21 proteins. 6 × His-Rpn8 and BSA are shown as negative controls, whereas histone H3 is used as a positive control. IB: Immunoblot. 32P: phosphate radiolabeled PAR.

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