Figure 7
Knockdown of H1 RNA inhibits the recruitment of EGFP-Rpp29 and EGFP-Rpp21 to laser-microirradiated sites. (A) TaqMan-based Real-Time PCR shows ~65% knockdown of H1 RNA. RNA was extracted from U2OS cells transfected with either a scramble shRNA or H1 RNA shRNA#165. Real-time PCR was performed to measure H1 RNA level. The y-axis represents the relative RNA level of H1 RNA, which was normalized to that of GAPDH. Error bars represent the SEM from two independent experiments. (B,C) Recruitment of EGFP-Rpp29 (B) and EGFP-Rpp21 (C) to DNA damage sites cells with shRNA knockdown of H1 RNA or control cells expressing a scramble shRNA. Graphs display the percentage of cells exhibiting accumulation of the two fusion proteins at DNA damage sites. Error bars show the SEM from two independent experiments and represent at least 30 different cells. P-values were calculated by two-sided Student’s t-test relative to scramble shRNA; ** and *** indicates significance of p < 0.01 and 0.001.
