Figure 5
Colocalization of ERG with ER stress sensors Atf6, Ire1α and ER chaperone protein Grp78/BiP by indirect immunofluorescence in 9 month-old Tg-ERG mouse prostate glands. Immunolocalization of Atf6 reveals the presence of activated Atf6 in the nucleus of Tg-ERG prostate luminal epithelial cells along with colocalized ERG (white arrows in the lower panel of A). Ire1α expression was increased in the Tg-ERG mouse prostates, correlating well with the colocalized expression of ERG in the luminal epithelial cells (white arrows in the lower panel of B). ER resident chaperone protein Grp78/BiP, localized mainly in the cytoplasm of the luminal epithelial cells was increased in Tg-ERG mouse prostates (white arrows in the lower panel of C).
