Figure 9
Spontaniously immortalized Tg-ERG mouse prostate epithelial cell line and epithelial cells isolated from Tg-ERG derived prostate spheres show similar features. Spontaniously immortalized Tg-ERG mouse prostate epithelial cell line, MoE1 (p6) grown in PrEGM supplemented with 1nM R1881 (A–E) show expression of both Ar (B) and ERG (C) by immunofluorescence using AR and ERG antibodies. Dapi (D) and Ar + ERG merged images (E) show colocalization of Ar and ERG in the muclei of MoE1 cells. Western blot analysis of MoE1 cells grown in charcoal stripped serum containing media show the expression of ERG upon induction with 1 nM R1881 (F). FACS analysis based on EpCAM of cells dissociated from the prostate spheres grown from wild-type, Tg-ERG mouse prostates in the absence (G i, ii) and presence of 10 nM DHT (G iii, iv) showabout 2–3 fold increase in EpCAM negative cell population (10.2% wt vs 27.4% Tg-ERG spheres). Consistant with prostate spheres, MoE1 cells (H) show increasing number of EpCAM (~97%).
