Figure 1 | Scientific Reports

Figure 1

From: Characterisation of a cell-free synthesised G-protein coupled receptor

Figure 1The alternative text for this image may have been generated using AI.

Overlap PCR of linear PCR expression templates and analytical scale CFPS test expression. (A) The various expression tags, containing all necessary regulatory elements were generated by an initial PCR. The expression tag, along with the PCR product of en2NTS1 was used to generate the full-length PCR product with the all necessary regulatory elements required for in vitro expression. (B) Expression was assessed by immunoblot against the His-tag found at the C-terminus of en2NTS1. The malE-signal sequence was identified as the best candidate for expression by CFPS.

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