Figure 4
From: Characterisation of a cell-free synthesised G-protein coupled receptor
Streptavidin and His-tag pull down assay of CFPS malE-en2NTS1 and E. coli expressed en2NTS1. Binding assay, testing capability of binding to Biotin-NT using Streptavidin dynabeads in combination with (A). CFPS produced malE-en2NTS1 and (B). The E. coli expressed en2NTS1 control. Each graph reads 1. Receptor alone. 2. Biotin-NT plus receptor. 3. Biotin NT, receptor and unlabelled NT8–13 competitor. Binding assay using His-tag isolation dynabeads in combination with (C). CFPS produced malE-en2NTS1 (D). E. coli expressed en2NTS1. Each graph reads 1. Receptor alone. 2. A647-NT8–13 plus receptor. 3. A647-NT8–13, receptor and NT8–13 competitor. Data is presented as triplicate experiments ± SEM.
