Table 1 Percentage composition of secondary structure for malE-en2NTS1 and E. coli produced en2NTS1 compared to structural data using pdb data inputted into STRIDE.

From: Characterisation of a cell-free synthesised G-protein coupled receptor

Data sets

CD data/CDpro output Algorithm: CDSSTR

CD data/CDpro output Algorithm: CONTIN-LL

CD data/CDpro output Algorithm: CDSSTR

CD data/CDpro output Algorithm: CONTIN-LL

STRIDEa analysis of NTS1-OGG7

 

malE-en2NTS1

malE-en2NTS1

en2NTS1

en2NTS1

NTS1-OGG7 (4BV0)

α-helix total (%)

60.5

58.6

56.1

69.3

63.1

β-strand total (%)

13

8.4

16

1

2.7

Turns (%)

13.8

23.2

13.8

4.4

7.2

Unordered (%)

10.9

9.7

14.3

25.3

6.4

Unknown (%)

—

—

 

—

20.6

nRMSD

0.03

0.106

0.08

0.224

—

  1. aSTRIDE calculations were based on the NTS1-OGG7 structure with the pdb accession code 4BV0. 4BV0 is a stable NTS1 variant lacking most of ICL3, and most of the N- and C-terminus. In addition only 297 residues of NTS1-OGG7 were identifiable compared to 374 residues of malE-en2NTS1. Therefore, for this analysis, secondary structure content was calculated for 374 residues resulting in missing information for 20.6% of the protein.
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