Figure 4
Overexpression of miR-125b-5p decreased the number and length of RBC ectopic dendrites and the ERG responses in RCS rats. Immunostaining of PKCα (red) and GFP (green) in the retinae of P60 RCS rats, five weeks after subretinal injection of (a) AAV-control or (b) AAV-125b virus. Bottom sub-panel shows magnification of the dotted area in the top left subpanel. Arrows indicate ectopic dendrites. (c) Number of RBC ectopic dendrites in RCS rats, five weeks after AAV-Control or AAV-125b treatment, per field view (213 µm × 213 µm) (bars show means; error-bars, SEM; n = 5). (d) Same for length of ectopic dendrites (n = 5) (e) Western blot analysis of BDNF expression in retinae of RCS rats after AAV-Control or AAV-125b treatment, five weeks post-surgery (bars, means; error-bars, SD, n = 4). GAPDH was used as a loading control. For original blot images, please see Supplementary Fig. 12. (f) Representative ERG traces in RCS rats, five weeks after subretinal injection of AAV-control or AAV-125b. (g) Group data for the amplitude of a-waves (top) and b-waves (bottom) from ERG recordings in RCS rats, five weeks after surgery (bars, means; error-bars, SD; n = 13). ONL: outer nuclear layer; OPL: outer plexiform layer; INL: inner nuclear layer; GCL: ganglion cell layer. *p < 0.05; #p < 0.01.
