Figure 2
From: Chlamydia trachomatis neither exerts deleterious effects on spermatozoa nor impairs male fertility
ROS production and lipid peroxidation in human spermatozoa exposed to CT. Analysis of ROS production by human spermatozoa after 6 h of in vitro incubation without bacteria (Vehicle), 100 nM PMA (positive control) or with increasing concentrations of EBs of CT serovar E or serovar LGV per million spermatozoa. ROS production was assessed by flow cytometry using the probe DCFH-DA that fluoresces when oxidized to DCFH. (a) Histograms show sperm with ROS production (M1). (b) ROS production levels (MFI) are shown in bars. (c) Human sperm membrane lipid peroxidation (%) after 6 h of in vitro incubation without bacteria (Vehicle), 100 mM TBHP (positive control) or with increasing concentrations of EBs of CT serovar E or serovar LGV per million spermatozoa. Lipid peroxidation was analyzed using BODIPY C11. Data are shown as mean ± SD. Fractions of sperm samples (n = 36) were tested separately. Statistical analysis was performed using one-way ANOVA with Bonferroni post hoc test analysis. *p < 0.05.
