Table 2 Effects of CT on the mitochondrial physiology and DNA integrity of human sperm.

From: Chlamydia trachomatis neither exerts deleterious effects on spermatozoa nor impairs male fertility

Sperm mitochondrial membrane potential, ΔΨm (%)a

CT

Serovar E

Serovar LGV

Sample

Vehicle

Control+

1 × 105 EBs

1 × 106 EBs

1 × 107 EBs

Vehicle

Control+

1 × 105 EBs

1 × 106 EBs

1 × 107 EBs

High ΔΨm

54.2 ± 3.8

45.2 ± 2.9*

55.0 ± 3.9

55.7 ± 8.4

51.1 ± 6.9

63.3 ± 6.2

48.3 ± 6.5*

67.5 ± 15.5

59.7 ± 5.4

58.3 ± 6.8

Low ΔΨm

20.1 ± 6.5

7.7 ± 2.2*

21.1 ± 4.4

19.1 ± 8.3

23.0 ± 7.8

16.2 ± 4.5

6.5 ± 2.7*

12.3 ± 7.0

20.0 ± 8.3

20.0 ± 7.3

Sperm DNA fragmentation b

DNA fragmentation index

4.0 ± 1.0

15.6 ± 0.7*

4.2 ± 0.6

4.3 ± 2.2

3.5 ± 2.6

3.1 ± 2.6

17.1 ± 1.1*

4.1 ± 1.3

5.3 ± 3.6

2.9 ± 1.3

  1. Percentages of human sperm exhibiting high and low mitochondrial membrane potential (ΔΨm)a, and evaluation of DNA fragmentationb, in human sperm samples after 6 h of in vitro incubation without bacteria (Vehicle), or with increasing concentrations of EBs of CT serovar E or serovar LGV. Data are shown as mean ± SD, n = 36. Statistical analysis was performed using one-way ANOVA with Bonferroni post hoc test analysis. *p < 0.05.
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