Figure 4

Histological analysis of neuron degeneration and apoptosis in the CA1 hippocampal region in acute status epilepticus mice. The brains were fixed with formalin at 4 h after anti-HMGB1, control IgG or PBS administration, and paraffin-embedded sections were stained with hematoxylin-eosin. Arrows indicate typical pyknotic cells. Scale bars equal 50 μm. *p < 0.05, **p < 0.01 compared with the sham. ^&&P < 0.01 compared with the PBS control. ^#p < 0.05 compared with the control IgG group (a). The number of pyknotic cells was counted in each mouse and the results are shown as the means ± SEM of 8 mice (b). Apoptotic cells were detected by TUNEL staining. The arrows indicate the apoptotic cells (c). The number of apoptotic cells in the CA1 region was counted in each mouse and the results are shown as the means ± SEM of 8 mice. **p < 0.01 compared with the sham control. ^&&p < 0.01 compared with the PBS control. ^##p < 0.01 compared with the control IgG group (d).