Table 1 Culture systems for sheep hepatocytes.
From: Hanging Drop, A Best Three-Dimensional (3D) Culture Method for Primary Buffalo and Sheep Hepatocytes
Culture system | Type of 3D culture | Media | First three days | Next Nine days |
|---|---|---|---|---|
Collagen-coated dish with Hepatozyme-SFM media (CH) | 17 µg/cm2 collagen coated* | Hepatozyme-SFM | Media + 10% FBS | Media |
Collagen-coated dish with William’s E media (CW) | 17 µg/cm2 collagen coated* | William’s E | Media + 10% FBS | Media |
polyHEMA-coated plates with Hepatozyme-SFM media (PH) | 12 mg/ml polyHEMA coated | Hepatozyme-SFM | Media + 10% FBS | Media |
polyHEMA-coated plates with William’s E media (PW) | 12 mg/ml polyHEMA coated | William’s E | Media + 10% FBS | Media |
Collagen sandwich with Hepatozyme-SFM media (SH) | Collagen sandwich | Hepatozyme-SFM | Media + 10% FBS | Media |
Collagen sandwich with William’s E media (SW) | Collagen sandwich | William’s E | Media + 10% FBS | Media |
Hanging drops with Hepatozyme-SFM media (HDH) | Hanging drop | Hepatozyme-SFM | Media + 10% FBS | Media |
Hanging drops with William’s E media (HDW) | Hanging drop | William’s E | Media + 10% FBS | Media |
