Table 4 Properties of the dominant bacteria at the genus level.
Phylum | Class | Order | Family | Genus | Characteristics56 |
|---|---|---|---|---|---|
Static & Dynamic water flow conditions | |||||
 B | Bacteroidia | Bacteroidales | Rikenellaceae | Unclassified | Gram-negative. Nonmotile. Anaerobic. |
 B | WCHB1-32 | Norank |  |  | Phylum is a phenotypically diverse group of Gram-negative rods. |
B | Sphingobacteriia | Sphingobacteriales | WCHB1-69 | Norank | Usually nonmotile. Aerobic or facultatively anaerobic. Limited fermentative capabilities are observed in some members. |
 F | Clostridia | Clostridiales | Peptococcaceae | Desulfosporosinus | Gram-negative. Motile. Strictly anaerobic. Sulfate and thiosulfate are reduced to sulfide in the presence of lactate but not in the presence of acetate or fructose. Incomplete oxidation of organic compounds to acetate. Acetate is the fermentation end product. Autotrophic growth with hydrogen plus sulfate. |
 F | Bacilli | Lactobacillales | Streptococcaceae | Lactococcus | Gram-positive. Nonmotile. Facultatively anaerobic; catalase-negative. Chemoorganotroph. Fermentative metabolism. |
P | γ-Proteobacteria | Aeromonadales | Aeromonadaceae | Aeromonas | Most species are motile. Facultatively anaerobic. Chemoorganotrophic, displaying oxidative and fermentative metabolism of D-glucose. Nitrate is reduced to nitrite. Usually oxidase- and catalase-positive. |
Only static water conditions | |||||
 B | Bacteroidia | Bacteroidales | Porphyromonadaceae | Paludibacter | Gram-negative. Nonmotile. Strictly anaerobic. Chemoorganotrophic. Oxidase- and catalase-negative. |
 B | Bacteroidia | Bacteroidales | Rikenellaceae | vadinBC27_wastewater -sludge_group | Gram-negative. Nonmotile. Anaerobic. |
 F | Clostridia | Clostridiales | Clostridiaceae | Clostridium _sensu_stricto_1 | Gram-positive. Most species are obligately anaerobic. Usually chemoorganotrophic; some species are chemoautotrophic or chemolithotrophic. Some species fix atmospheric nitrogen. Do not carry out a dissimilatory sulfate reduction. Usually catalase-negative. |
 F | Clostridia | Clostridiales | Gracilibacteraceae | Gracilibacter | Gram-positive cell-wall structure, but stains Gram-negative. Obligately anaerobic, chemoorganotrophic. |
 F | Clostridia | Halanaerobiales | Halobacteroidaceae | Uncultured | Gram-negative. Strictly anaerobic. Oxidase- and catalase-negative. Most species ferment carbohydrates. Some species may grow fermentatively on amino acids; others have a homoacetogenic metabolism or grow by anaerobic respiration while reducing nitrate, trimethylamine N-oxide, or selenate. |
 P | β-Proteobacteria | Burkholderiales | Comamonadaceae | Acidovorax | Gram-negative. Motile. Aerobic, having a strictly oxidative type of metabolism with oxygen as the terminal electron acceptor. Oxidase positive. Chemoorganotrophic. |
 P | β-Proteobacteria | Burkholderiales | Comamonadaceae | Comamonas | Gram-negative. Motile. Aerobic. Oxidase- and catalase-positive. Chemoorganotrophic, oxidative carbohydrate metabolism with oxygen as the terminal electron acceptor. |
 P | γ-Proteobacteria | Pseudomonadales | Pseudomonadaceae | Pseudomonas | Gram-negative. Motile by one or several polar flagella; rarely nonmotile. Aerobic, having a strictly respiratory type of metabolism with oxygen as the terminal electron acceptor; in some cases nitrate can be used as an alternate electron acceptor, allowing growth to occur anaerobically. Oxidase-positive or -negative. Catalase-positive. Chemoorganotrophic. |
Only dynamic water flow conditions | |||||
 F | Clostridia | Clostridiales | Eubacteriaceae | Alkalibacter | Gram-positive. Nonmotile. Strictly anaerobic, catalase negative. Chemoorganoheterotroph. |
 F | Clostridia | Clostridiales | Christensenellaceae | R-7_group 57 | Gram-negative. Anaerobic. |
 F | Clostridia | Clostridiales | Incertae Sedis | Sedimentibacter | Gram-positive or -negative. Motile. Strict anaerobe. |
 P | β-Proteobacteria | Burkholderiales | Comamonadaceae | Unclassified | Gram-negative. Chemoorganotrophic or facultatively chemolithotrophic with hydrogen or carbon monoxide oxidation. Possess a strict respiratory type of metabolism, with oxygen as the terminal electron acceptor. Some species can also use nitrates. Oxidase positive. |
 P | β-Proteobacteria | Rhodocyclales | Rhodocyclaceae | 12up | Family is phenotypically, metabolically, and ecologically diverse. Includes photoheterotrophs; aerobes, anaerobes, and facultative anaerobes utilizing a number of electron acceptors; fermentative organisms; and nitrogen-fixing organisms. |
 P | β-Proteobacteria | Rhodocyclales | Rhodocyclaceae | Zoogloea | Gram-negative. Actively motile. Aerobic, having a strict respiratory type of metabolism with oxygen or nitrate as the terminal electron acceptor. Denitrification occurs with the formation of nitrogen. Oxidase-positive. Weakly catalase-positive. Chemoorganotrophic. |
 P | γ-Proteobacteria | Enterobacteriales | Enterobacteriaceae | Enterobacter | Gram-negative. Motile. Facultatively anaerobic. Glucose is fermented with the production of acid and gas (generally carbon dioxide:hydrogen = 2:1). Nitrate is reduced to nitrite. Hydrogen sulfide is not produced from thiosulfate. |
 P | δ-Proteobacteria | Desulfobacterales | Desulfobulbaceae | Desulfocapsa | Motile. Strictly anaerobic. Simple organic compounds are incompletely oxidized, with sulfate as electron donor that is reduced to sulfide. Support chemolithoautotrophic growth with carbon dioxide as carbon source. Occur in sediments of freshwater or marine habitats close to the anoxic/oxic interface. |
