Figure 2
Determining and confirming the sensitivity of S. cerevisiae cells to PCA. (A) Determining the sensitivity of S. cerevisiae cells to 50 μg/ml PCA. Cellular OD600 was measured with an iMark microplate reader for cells at initial OD600 = 0.1 in YPD treated with or without 50 μg/ml PCA at the indicated times (intervals of 2 h, to 12 and 24 h). Cells in YPD without additive (−) or with acetone only (+0) were used as controls. YEL, yeast essential library. The full names of specific mutants with obvious sensitivity changes are given on the figure. (B) Confirming the sensitivity of S. cerevisiae cells to 20 μg/ml PCA. The screened mutants with obvious changes in resistance or sensitivity to PCA, determined with the method shown in panel A, were cultured in YPD to log phase and further tested in YPD without additive (upper), YPD with acetone only (middle) and YPD with 20 μg/ml PCA (bottom) to confirm their responses to PCA.
