Figure 4

Mutants defective in vacuolar ATPase and protein sorting show increased sensitivity to PCA. (A) Overnight YPD-cultured wild-type and vacuolar ATPase-mutant cells were spotted onto YPD plates containing 0 or 60 μg/ml PCA and incubated for 2 days at 26 °C. (B) Overnight YPD-cultured wild-type and protein sorting-mutant cells tagged with GFP-Snc1 on the chromosome were spotted onto YPD plates containing 0 or 60 μg/ml PCA and incubated for 2 days at 26 °C. (C) Wild-type and protein sorting-mutant cells tagged with GFP-Snc1 on the chromosome were grown to mid-log phase in YPD for 5 h to examine GFP-Snc1 localization. White arrowheads indicate cells in which the polar distribution of GFP-Snc1 was lost. The bar represents 5 μm.