Figure 7 | Scientific Reports

Figure 7

From: A genetic screen in combination with biochemical analysis in Saccharomyces cerevisiae indicates that phenazine-1-carboxylic acid is harmful to vesicular trafficking and autophagy

Figure 7

Treatment with PCA alters the autophagy process differently than hygromycin B. (A) HYG treatment did not disrupt the localization of GFP-Atg8. (B) HYG treatment slightly retarded GFP-Atg8 processing in a dose-dependent manner in SD-N. (C) Quantification of blots in panel B. (D) PCA treatment altered the localization of GFP-Atg8 in YPD and SD-N differently. (E) PCA treatment induced slight autophagy at low PCA concentrations in YPD and impaired autophagy in SD-N. (F) Quantification of blots in panel E. PhC, phase contrast. The bar represents 5 μm. Arrows indicate cells with GFP inside the vacuoles. Arrowheads indicate the ER ring inside the cell. The results shown represent two independent experiments.

Back to article page