Figure 5
From: Identification of a nanobody specific to human pulmonary surfactant protein A

Immunofluorescent staining and immunehistochemical staining with Nb4. (a) Human epithelial A549 cells were positively stained with Nb4. Human lung epithelial A549 cells, human liver L-02 cells, and human kidney 293 T cells were fixed and stained with SP-A-mAb, Nb4-FITC, and off-target Nb (negative). (b) Human lung tissues were positively stained with Nb4. Human lung, liver, spleen, and kidney tissue specimens were stained with SP-A-mAb, Nb4-His, and H1N1-His (off-target control).