Table 1 The fragments of sculptin generated by factor Xa.

Fragment number^a	Fragment of sculptin^b	Theoretical Average Mass	Calculated Average Mass^c
H1^d	GKPQGHPHDALEARSDAVHTAVPKMPKGGHGGFEPIPIDYDERALEAR	5156.74	5153.57
H2^d	SDAVHTAVPKMPKGGHGGFEPIPIDYDERALEAR	3663.10	3667.50
H2^d	SDAVHTAVPKMPKGGHGGFEPIPIDYDERALHALEHHHHHH	4579.05	4582.40
H3^d	SDAVHTAVPKMPKGGHGGFEPIPIDYDERALEARSDAVHTAVPKMPKGGHGGFEPIPIDYDERALHALEHHHHHH	8223.13	8220.55
H3^d	SDAVHTAVPKMPKGGHGGFEPIPIDYDERALEARSDAVHTAVPKMPKGGHGGFEPIPIDYDER	6765.55	6770.60
H4^d	SDAVHTAVPKMPKGGHGGFEPIPIDYDERALEARSDAVHTAVPKMPKGGHGGFEPIPIDYDERALEAR	7306.17	7299.61
H4^d	GKPQGHPHDALEARSDAVHTAVPKMPKGGHGGFEPIPIDYDERALEARSDAVHTAVPKMPKGGLGGFEPIPIDYDERALEARSDAVHTAVPKMPKGGHGGFEPIPIDYDERALEAR	12420.90	12427.54

Sculptin (10 µM) was incubated without or with 1 µM of factor Xa in 50 mM phosphate buffer containing 150 mM NaCl and 50 µM PS/PC, pH 7.4 for 6 h at 37 °C. The reaction mixtures were separated by reverse phase C-18 HPLC column. The peaks were subjected to MALDI-TOF MS.
^aPeak number of the HPLC chromatogram (Fig. 5A).
^bFragment of sculptin generated by factor Xa.
^cThe experimental average mass calculated by MALDI-TOF mass spectrometry (Fig. 5B).
^dThe fractions (HPLC peaks) were subjected to thrombin inhibition test (Fig. 5C,D).

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