Figure 1 | Scientific Reports

Figure 1

From: Construction of a highly efficient CRISPR/Cas9-mediated duck enteritis virus-based vaccine against H5N1 avian influenza virus and duck Tembusu virus infection

Figure 1

Schematic illustration of the novel candidate trivalent vaccine C-KCE-HA/PrM-E development. (A) Full-length of the attenuated commercial DEV vaccine strain (C-KCE). (B) Two portions of the genome C-KCE expanded to show the UL27, UL26, US7, and US8, and the gene junction regions are depicted. (C) The organization of transfer fragments Left arm-RFP-Right arm and Left arm-GFP-Right arm. (D) The recombinant C-KCE-RFP/GFP was generated using CRISPR/Cas9 system. (E) The organization of transfer fragments Left arm-HA-Right arm and Left arm-PrM-E-Right arm. (F) Following the CRISPR/Cas9 system mediated recombination, the recombinant C-KCE-HA/PrM-E was generated.

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