Figure 5
From: A robust in vitro model for trans-lymphatic endothelial migration
Adhesion molecule expression and CD4 T cell migration across SVEC4-10 layers treated with TNFα, IFNγ, or S1P. (A) SVEC4-10 or mLEC treated with TNFα and/or IFNγ, stained for VCAM-1 and ICAM-1, and analyzed with flow cytometry. (B) Fluorescent staining of SVEC4-10 treated with medium, TNFα or S1P for VCAM-1 and VE-cadherin (left) and quantitation of area labeled (right), x20, scale bar 42 μm. (C) Fluorescent staining of SVEC4-10 layers treated with medium, TNFα or S1P for VCAM-1, moesin and β-catenin (left) and quantitation of label clustering (right), x20, scale bar 42 μm. (D) CD4 T cell migration across iSVEC4-10 treated with TNFα, and blocked with anti-VLA-4 and/or anti-VCAM-1 mAbs. Representative of three independent experiments with triplicate wells in each assay *P < 0.05.
