Figure 6

Diverse cell types migrate in a chemokine-driven vectorial fashion across LEC. (A–D) Activated CD4 T cells (A), memory CD4 T cells (CD4⁺CD44^hiFoxp3-GFP⁻) (B), DC (CD11c⁺MHCII⁺) (C), and mature DC (CD11c⁺MHCII⁺CD86^hiCD80^hi) (D) migration across iSVEC4-10 or SVEC4-10 toward CCL19. (E) Peritoneal macrophages migration toward CCL2 and CCL20 across iSVEC4-10 or SVEC4-10. (F) CD8 T cells migration across iSVEC4-10 or SVEC4-10 toward CCL19. (G) Human breast carcinoma lines MCF7 (left) and MDA-MB-231 (right), migration across hLEC and ihLEC toward S1P and CXCL12. (H,I) Mouse breast cancer cell lines 410 (H) and 410.4 (I) migration across SVEC4-10, iSVEC4-10, toward S1P or CXCL12. Representative of three independent experiments with triplicate wells in each assay *P < 0.05.