Figure 2
From: Benchmarking of RNA-sequencing analysis workflows using whole-transcriptome RT-qPCR expression data
The overlap of the rank outlier genes between samples (MAQCA and MAQCB) and workflows is significant. (A) The number of genes with an (absolute) rank shift of more than 5000 are indicated. Genes marked as down have a higher expression rank in RT-qPCR, genes marked as up have a higher expression rank in RNA-seq. (B) The overlap of genes with an absolute rank shift of more than 5000 between MAQCA and MAQCB is significant for each workflow (Fisher exact test) (C) The overlap of the genes with an absolute rank shift of more than 5000 between the different workflows is significant (Super exact test). (D) Genes with an absolute rank shift of more than 5000 have an overall lower expression. The Kolmogorov-Smirnov p-value for the intersection of rank outlier genes between methods is shown. Results are based on RNA-seq data from dataset 1.
